True Blue Organics Hand Sanitiser is laboratory tested and passes conventional bacterial tests.The method employed to carry out this testing was based on AOAC Official Method 960.09 (Germicidal and Detergent Sanitizing Action of Disinfectants).
The neutraliser buffer used was DE Neutralising Broth.
The organisms used were:
• Escherichia coli
• Staphylococcus aureus
• Pseudomonas aeruginosa
• Candida albicans
Numeric counts were performed after one minute and five minute contact times.
A numeric count of the inoculant solutions was carried out before any contact times to ascertain the theoretical level of organisms inoculated into each sample. This is shown in the table below.
The sanitiser shows good effectiveness against Gram negative organisms (E.coli and
Pseudomonas) after one minute exposure time.
The neutraliser buffer used was DE Neutralising Broth.
The organisms used were:
• Escherichia coli
• Staphylococcus aureus
• Pseudomonas aeruginosa
• Candida albicans
Numeric counts were performed after one minute and five minute contact times.
A numeric count of the inoculant solutions was carried out before any contact times to ascertain the theoretical level of organisms inoculated into each sample. This is shown in the table below.
| Organism | cfu per mL of inoculum | Theoretical cfu per mL/g of Product |
|---|---|---|
| Escherichia coli | 880,000,000 | 8,800,000 |
| Staphylococcus aureus | 550,000,000 | 5,500,000 |
| Pseudomonas aeruginosa | 1,600,000,000 | 16,000,000 |
| Candida albicans | 56,000,000 | 560,000 |
Results of the trial can be seen in the tables below.
Escherichia coli Test
| Contact time | Count before contact | LOG10 | Count after contact | LOG10 | LOG10 Decrease | % Decrease |
|---|---|---|---|---|---|---|
| 1 minute | 8,800,000 | 6.94 | <100 | <2 | >4.94 | >99.999 |
| 5 minutes | 8,800,000 | 6.94 | <100 | <2 | >4.94 | >99.999 |
Staphylococcus aureus Test
| Contact time | Count before contact | LOG10 | Count after contact | LOG10 | LOG10 Decrease | % Decrease |
|---|---|---|---|---|---|---|
| 1 minute | 5,500,000 | 6.74 | 1,800,000 | 6.26 | 0.48 | 67.27 |
| 5 minutes | 5,500,000 | 6.74 | 300 | 2.48 | 4.26 | 99.995 |
Pseudomonas aeruginosa Test
| Contact time | Count before contact | LOG10 | Count after contact | LOG10 | LOG10 Decrease | % Decrease |
|---|---|---|---|---|---|---|
| 1 minute | 16,000,000 | 7.20 | <100 | <2 | >5.20 | >99.999 |
| 5 minutes | 16,000,000 | 7.20 | <100 | <2 | >5.20 | >99.999 |
Candida albicans Test
| Contact time | Count before contact | LOG10 | Count after contact | LOG10 | LOG10 Decrease | % Decrease |
|---|---|---|---|---|---|---|
| 1 minute | 560,000 | 5.75 | 120,000 | 5.08 | 0.67 | 78.57 |
| 5 minutes | 560,000 | 5.75 | <100 | <2 | >3.75 | 99.98 |
Pseudomonas) after one minute exposure time.
Effectiveness against Gram positive organism (Staphylococcus) and yeast (Candida)
is somewhat less effective, with reasonable effectiveness only visible after 5 minutes.
Organic Hand Sanitiser - Protection for Everyone's Skin