Tea Tree Oil Chromatographic Profile
The chemical composition of Tea Tree oil is defined by the International Standard for Tea Tree oil (ISO 4730:2004) entitled “Oil of Melaleuca terpinen-4-ol type”. This specifies levels of 15 of the more than 100 components in pure Australian tea tree oil.
Results of Chromatographic Profile by Chemical Ecology and Essential Oil Research Unit at Primary Industries Institute, Wollongbar, Australia
Australian Tea Tree Oil - Terpinen-4 - Ol Type
Method Used - Area Normalisation Based on Iso 4730: 2004
|1. α-Piniene||2.1||1 - 6|
|2. Sabinene||0.1||tr - 3.5|
|3. α-Terpinene||8.2||5 - 13|
|4. Limonene||1.5||0.5 - 1.5|
|5. p-Cymene||1.7||0.5 - 8|
|6. 1,8-Cineole||8.7||tr - 15|
|7. γ-Terpinene||17.8||10 - 28|
|8. Terpinolene||2.9||1.5 - 5|
|9. Terpinen-4-ol||43.2||30 - 48|
|10. α-Terpineol||3||1.5 - 8|
|11. Aromadendrene||0.7||tr - 3|
|12. Ledene||0.5||tr - 3|
|13. δ-Cadinene||0.6||tr - 3|
|14. Globulol||0.4||tr - 1|
|15. Viridiflorol||0.3||tr - 1|
|PS: This oil smells like a typical tea tree oil with a slight eucalyptus finish.|
Tea Tree Oil Certificate of Analysis
1. COMPANY DETAILS
1.1 Company: True Blue Organics
1.2 Address: 4545 Karamea Highway, R.D.3, Karamea 7893, New Zealand
1.3 Telephone Number: +64 3 7826 888
1.4 Fax Number: +64 3 7826 088
1.5 Email: See Contact Details
1.6 Web Site: www.trueblueorganics.co.nz
2. PRODUCT IDENTIFICATION AND COMPOSITION
2.1 Product Name: Tea Tree Oil: Melaleuca oil: Oil of Melaleuca – terpinen-4-ol type
2.2 Botanical Name: Melaleuca Alternifolia
2.3 Product Code: TBO TEATREE
2.4 Chemical Abstract number: 68647
2.5 Country of Origin: New Zealand
2.6 Expiry Date: October 2010
2.7 USA INCI Name: Melaleuca Alternifolia [Tea Tree] Leaf Oil
2.8 EU INCI Name: Melaleuca Alternifolia
2.9 Natural essential oil steam distilled, 100% pure.
2.10 UN No. 2319
2.11 Tarrif code. 3301.29.00
2.12 Uses: Antibacterial; Natural Fragrance Ingredient.
3. PHYSICAL PROPERTIES
3.1 Appearance: Clear, colourless to pale yellow mobile liquid with no visible trace of water.
3.2 Odour: Characteristic warm, spicy odour
3.3 Refractive Index: @ 20°C: 1.4750 – 1.4820
3.4 Specific Gravity: @ 20°C: 0.885 – 0.906
3.5 Solubility: @ 20°C1 vol soluble in 2 vols 85% v/v ethanol
4. CHEMICAL PROPERTIES – Analysis By Gas Chromotography
Pharmaceutical Grade: 1,8 cineole < 4.5% terpinen-4-ol > 36%
Constituents as per International Standard – ISO 4730
TYPICAL ANALYSIS BY GC/MS: 1,8-CINEOL <2.4% TYPICAL, TERPINEN-4-OL 38% TYPICAL ISO 4730: α-PINENE (1-6%), SABINENE (TR.-3.5%), α-TERPINENE (5-13%), LIMONENE (0.5 4%), PCYMENE (0.5-12%), 1,8-CINEOL (0-15%), γ-TERPINENE (10-28%), TERPINOLENE (1.55%), TERPINEN-4-OL (30% +), α-TERPINEOL (1.5- 8%), AROMADENDRENE (TR-7%), LEDENE (VIRIDIFLORENE) (0.5-6.5%), δ-CADINENE (TR-8%) , GLOBULOL (TR-3%), VIRIDIFLOROL (TR-1.5%).
5. Standards and Monographs
Australian Standards AS 2782-1985 – Oil of Melaleuca Terpinen-4-ol type
International Standard ISO 4730 – Oil of Melaleuca Terpinen-4-ol type (Tea Tree oil)
British Pharmaceutical Codex, 1949 (pp 597-598)
Martindale, The Extra Pharmacopoeia, 28th edition, 1982 (678)
The Dispensatory of the United States of America, 25th edition, (p1610)
6. HAZARD IDENTIFICATION
Physical – Chemical Hazards
Flammable: Flammable liquid, produces oxides of carbon when burning.
Extinguishing Media: Dry Chemical Foam/CO²
Do not use water of aqueous systems
Special Fire Fighting Procedures: None
Unusual Fire and Explosion Hazards: None
Flammability and Explosive Data
Flash Point – Pensky Martens Closed Cup: 57° C – 60°C
Stability: Presents no significant reactivity hazard
Stable for at least 10 years if stored correctly
Subject to oxidation if stored incorrectly
Incompatibility: Solvent – avoid contact with plastics, rubber, oil-based paints, ink etc.
Hazardous Polymerisation: Does not occur
Toxicity and Health Hazard Data:
Toxicity: May produce toxic effects if taken internally
Irritation: Draize Skin Irritancy Index 5.0 – could cause dermatitis in some users.
Sensitisation: Nil skin sensitisation in test animal
Ames Test: Not mutagenic
Health Hazards: If taken internally the toxic effects which may be experienced include seizures, coma and respiratory depression.
Rapid adsorption may be expected
Eye Contact: Irrigate with water
Ingestion: Do not induce vomiting
Give a glass of water
If poisoning occurs, get to a doctor or hospital quickly
Personal Protection Data:
Respiratory: None required
Ventilation: Good room ventilation required
Eye Protection: Safety glasses optional
Other Protective Equipment: None required
Preservative Challenge Tests:
Passes USP and B.P. Preservative challenge tests at 0.8% (v/v)
Conditions: Store in cool, dry place in tight sealed, suitably sized container and protect from light
Storage and handling apparatus should not contain plastic or rubber components
Epoxy phenolic lined steel drums (shipping or short-term storage only)
Polyethylene (shipping or short-term storage only)
Packaging Code: Flammable liquid Class 3
UN No: 2319
Name and Description – Terpene Hydrocarbons, N.O.S. Class 3
Subsidiary risk – nil
UN Packaging group lll
Limited Quantities – 5 Litres
Packaging and IBCs: Packing Instructions – P001, IBC03, LP01
Portable Tank Instruction T4
Portable tank special provisions – TP1, TP29
Aluminium Bottles – Universal Packaging = ISO 9001
Lacquer lined steel/tin Moderate
Other plastic Yes
Stainless steel drums preferred.
When stored within advised conditions, re-test after 12 months, then at 3 monthly intervals up to a
maximum life of 36 months.
7. SAFE HANDLING AND STORAGE INFORMATION
Avoid inhalation and contact with skin and eyes. Good personal hygiene practices should be used. Wash after any contact, before breaks and meals, and at the end of the work period. Contaminated clothing and shoes should be
cleaned before re-use.
Hazardous material. A Scheduled 6 Poison.
Containers should be kept closed in order to minimise contamination.
Store in a cool, dry, well ventilated place protected from light.
Keep from extreme heat and away from ALL sources of ignition.
8. STABILITY AND REACTIVITY
Chemically stable, non hazardous material.
This material presents no significant reactivity hazard.
8.2 Conditions to Avoid:
8.3 Materials to Avoid:
Strong acids, strong alkalis and oxidizing agents.
9. ECOLOGICAL INFORMATION
None established. Avoid pollution to soil, rivers and the ocean.
Low mobility in the soil.
9.3 Persistence & Degradability:
Low persistence level and readily biodegradable.
9.4 Bio-accumlative Potential:
10. DISPOSAL CONSIDERATION
10.1 Waste Material:
Place material and / or absorbent into sealed containers and dispose of in accordance with current applicable laws and regulations. Refer to local Authority advice.
Empty containers can have residues, gases and mists.
Use the proper Waste Disposal Methods & Procedures for Empties as well.
11.1 Classified as Dangerous Goods by the criteria of the Australian Dangerous Goods Code for Transport by Road and Rail. Transport as per the “Australian Dangerous Goods Code” for Class 3 [Flammable Liquids], Packaging Group III Substances.
Not to be transported with Classes 1 [Explosives], 2.1 [Flammable Gases] [nb this applies only when in bulk], 2.3 [Poisonous Gases], 4.2 [Spontaneously Combustible Substances], 5.1 [Oxidising Agents], 5.2 [Organic Peroxides], 7 [Radioactive Substances], and Foodstuffs or Foodstuff empties.
11.2 Proper Shipping Name:
TERPENE HYDROCARBONS, N.O.S.
11.3 UN Number: 2319
11.4 Packaging Group: III
11.5 DG Class: Class 3
11.6 Poison Schedule: Schedule 6 POISON.
11.7 Hazchem Code: 3[Y]
Tea Tree Oil is obtained from the leaves of the Tea Tree variety Melaleuca alternifolia, by the process of steam distillation. The essential oil is tested by gas chromatographic analysis in the Wollongbar Agricultural Institute laboratory, Australia, to ensure it conforms to the specifications of both the Australian Standards, AS 2782 –1985 – Oil of Melaleuca Terpinen-4-ol type and the International Standard ISO 4730 – Oil of Melaleuca Terpinen-4-ol type.
It is exclusively gained from non-GMO New Zealand grown tea trees and is free of any GMO-fragments.
The essential oil is certified organic by BioGro NZ. It carries full IFOAM accreditation which is accepted internationally as the highest organic standard.
The information contained in this Certificate of Analysis is obtained from current and reliable sources. True Blue Organics provides the information in good faith but makes no representation as to its comprehensiveness or accuracy. This Material Safety Data Sheet summarises our best current knowledge of the health and safety hazard information of the product. This document is thus intended only as a guide to the appropriate precautionary handling of the material by properly trained personnel using this product. Individuals receiving this information must exercise their independent judgment in determining its appropriateness for a particular purpose.
Document Prepared By: Margaret Macbeth, Owner and Manager.